Molecular Methods in Plant Disease Diagnostics : Principles and Protocols
CABI
2016
EISBN 9781780641485
Molecular Methods in Plant Disease Diagnostics; Copyright; Contents; Editors and Contributors; Foreword; 1: Introduction: advances in plant diagnostics – historical perspectives and future directions; 1.1 Progress in recent years; 1.2 The rise and rise of PCR; 1.3 Quality matters; 1.4 The move from laboratory to field; 1.5 Identifying the unknown; 1.6 Future challenges; References; 2: Conventional PCR; 2.1 Introduction; 2.2 Viruses and viroids; 2.3 Phytoplasmas; 2.4 Bacterial pathogens; 2.5 PCR detection of fungi; 2.6 Internal controls; 2.7 PCR optimization; 2.7.1 Primers
2.7.2 Cycling conditions2.7.3 Reaction components; 2.7.4 PCR additives or enhancers; Protocols; Protocol 2.1: One-step PCR to detect potyviruses (Marie-Jeanne et al., 2000); Materials; Method; Notes; Protocol 2.2: Nested-PCR to detect phytoplasmas; Materials; Method; Notes; References; 3: Real-time PCR; 3.1 Introduction; 3.2 Coupling PCR ampl
Using molecular methods for plant disease diagnosis provides diagnosticians with a number of advantages over more traditional methods. They can allow the identification of morphologically similar species, for example, or the detection of infection prior to symptom formation. Not only can molecular tools help by increasing the efficacy, accuracy and speed of diagnosis; their common technological basis provides further benefits, especially where resources are limited and traditional skills are hard to sustain. This book provides protocols for nucleic acid-based methods currently applied to plant pathogen detection and identification. It takes the practitioner through the full range of molecular diagnostic and detection methods and, as these generic techniques are appropriate for use on any target with minimal modification, also provides a useful resource for students of plant pathology and plant pathologists. Beginning with the background and future directions of the science, it then addresses DNA barcoding, microarrays, polymerase chain reactions (PCR), quality assurance and more, forming a complete reference on the subject.
2.7.2 Cycling conditions2.7.3 Reaction components; 2.7.4 PCR additives or enhancers; Protocols; Protocol 2.1: One-step PCR to detect potyviruses (Marie-Jeanne et al., 2000); Materials; Method; Notes; Protocol 2.2: Nested-PCR to detect phytoplasmas; Materials; Method; Notes; References; 3: Real-time PCR; 3.1 Introduction; 3.2 Coupling PCR ampl
Using molecular methods for plant disease diagnosis provides diagnosticians with a number of advantages over more traditional methods. They can allow the identification of morphologically similar species, for example, or the detection of infection prior to symptom formation. Not only can molecular tools help by increasing the efficacy, accuracy and speed of diagnosis; their common technological basis provides further benefits, especially where resources are limited and traditional skills are hard to sustain. This book provides protocols for nucleic acid-based methods currently applied to plant pathogen detection and identification. It takes the practitioner through the full range of molecular diagnostic and detection methods and, as these generic techniques are appropriate for use on any target with minimal modification, also provides a useful resource for students of plant pathology and plant pathologists. Beginning with the background and future directions of the science, it then addresses DNA barcoding, microarrays, polymerase chain reactions (PCR), quality assurance and more, forming a complete reference on the subject.
